However, some eukaryotic pyrogenic microorganisms are not detectable. When exposed to pyrogens secretion of interleukin-6 is induced. The monocytic cell line Mono Mac 6 is sensitive to pyrogens. Moesby, Lise Hansen, E W Christensen, J D Ultrasonication of pyrogenic microorganisms improves the detection of pyrogens in the Mono Mac 6 assay These results suggest that leukocyte pyrogen was synthesized de novo from amino acid precursors and that leukocytes made the same pyrogen whatever the stimulus used to activate them. Furthermore, pyrogens obtained from exudate cells stimulated in different ways, or from blood cells and exudate cells stimulated with endotoxin, appeared to be identical. All the purified pyrogens were radioactive and for all it was shown that radioactivity and pyrogenic activity coincided on electrophoresis at pH 3.5 and pH 9 in acrylamide and on isoelectric focusing in acrylamide.
The crude leukocyte pyrogen samples were purified pyrogen from exudate cells was rendered homogeneous pyrogen from blood cells was still contaminated with other proteins.
The stimuli used were endotoxin, phagocytosis, and tuberculin. Rabbit ieukocytes from peritoneal exudates and from blood were stimulated to form leukocyte pyrogen in the presence of radiolabeled amino acids. Moore, D M Murphy, P A Chesney, P J Wood, W B Synthesis of endogenous pyrogen by rabbit leukocytes. In its monomer form, endogenous pyrogen is a potent fever-producing substance and mediates fever by its action on the thermoregulatory center. Recent evidence suggests that following release, molecular aggregates form which are biologically active. Once synthesis is completed, the molecule is released without significant intracellular storage. Whatever the mechanism involved, endogenous pyrogen is synthesized following transcription of new DNA and translation of mRNA into new protein. Some tumors of macrophage origin produce the molecule spontaneously. Some of these agents stimulate production of endogenous pyrogen because they are toxic others act as antigens and interact with either antibody or sensitized lymphocytes in order to induce its production. Endogenous pyrogen is a low-molecular-weight protein released from phagocytic leukocytes in response to several substances of diverse nature. The production and release of endogenous pyrogen by the host is the first step in the pathogenesis of fever.
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